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Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis

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Title Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis
 
Creator FULSUNDAR, S
KULKARNI, HM
JAGANNADHAM, MV
NAIR, R
KEERTHI, S
SANT, P
PARDESI, K
BELLARE, J
CHOPADE, BA
 
Subject PROTEIN SUBCELLULAR-LOCALIZATION
GRAM-NEGATIVE BACTERIA
PROTEOMIC ANALYSIS
CARBAPENEM RESISTANCE
BIOFILM FORMATION
ESCHERICHIA-COLI
ANTIMICROBIAL SUSCEPTIBILITY
PSEUDOMONAS-AERUGINOSA
FUNCTIONAL-ANALYSIS
HEALTHY HUMANS
Acinetobacter radioresistens
OMV
Biofilm augmentation
Cytotoxicity
Apoptosis
 
Description Acinetobacter radioresistens is an important member of genus Acinetobacter from a clinical point of view. In the present study, we report that a clinical isolate of A. radioresistens releases outer membrane vesicles (OMVs) under in vitro growth conditions. OMVs were released in distinctive size ranges with diameters from 10 to 150 nm as measured by the dynamic light scattering (DLS) technique. Additionally, proteins associated with or present into OMVs were identified using LC-ESI-MS/MS. A total of 71 proteins derived from cytosolic, cell membrane, periplasmic space, outer membrane (OM), extracellular and undetermined locations were found in OMVs. The initial characterization of the OMV proteome revealed a correlation of some proteins to biofilm, quorum sensing, oxidative stress tolerance, and cytotoxicity functions. Thus, the OMVs of A. radioresistens are suggested to play a role in biofilm augmentation and virulence possibly by inducing apoptosis. (C) 2015 Elsevier Ltd. All rights reserved.
 
Publisher ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
 
Date 2016-01-15T04:38:48Z
2016-01-15T04:38:48Z
2015
 
Type Article
 
Identifier MICROBIAL PATHOGENESIS, 83-84,12-22
0882-4010
http://dx.doi.org/10.1016/j.micpath.2015.04.005
http://dspace.library.iitb.ac.in/jspui/handle/100/17778
 
Language en