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IR 820 dye encapsulated in polycaprolactone glycol chitosan: Poloxamer blend nanoparticles for photo immunotherapy for breast cancer

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Title IR 820 dye encapsulated in polycaprolactone glycol chitosan: Poloxamer blend nanoparticles for photo immunotherapy for breast cancer
 
Creator KUMAR, P
SRIVASTAVA, R
 
Subject LIPID-POLYMER NANOPARTICLES
INDOCYANINE GREEN
PHOTOTHERMAL THERAPY
DRUG-DELIVERY
APOPTOSIS
EFFICACY
CELLS
IR 820
Photothermal therapy
TNF-alpha
Poloxamer
Biocompatibility
Reactive oxygen species
 
Description In the present study, we have fabricated biocompatible and biodegradable monodisperse IR 820 encapsulated polycaprolactone (PCL) glycol chitosan (GC): Poloxamer blend nanoparticles (PP-IR NPs) for imaging and effective photo-immunotherapy. IR 820 has been used as an imaging and photothermal agent whereas glycol chitosan (GC) as an immunostimulatoty agent. The combination of IR 820, poloxamer, and GC can be used effectively for photoimmunotherapy for cancer, drug-resistant and TNF-alpha resistant estrogen positive breast cancer. PP-IR NPs are stable in aqueous solution. The uniform size of 100-220 nm with a high zeta value of +38 +/- 2 mV led them to accumulate in cancer cells. Laser treatment did not affect the morphology of PP-IR NPs as observed under the transmission electron microscope (TEM). In vitro cytotoxicity studies on MCF-7 cells showed enhanced toxicity upon laser treatment. Further, we validated the cell death by reactive oxygen species (ROS) production. Our studies thus showed that PP-IR NPs are effective in suppressing metastatic cancer as the combinational therapy leads to the formation of apoptotic bodies in MCF-7 cells. (C) 2015 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2016-01-15T04:44:25Z
2016-01-15T04:44:25Z
2015
 
Type Article
 
Identifier MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 57,321-327
0928-4931
1873-0191
http://dx.doi.org/10.1016/j.msec.2015.08.006
http://dspace.library.iitb.ac.in/jspui/handle/100/17789
 
Language en