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Competitive binding of Chlorin p(6) and Dansyl-L-Proline to Sudlow's site II of human serum albumin

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Title Competitive binding of Chlorin p(6) and Dansyl-L-Proline to Sudlow's site II of human serum albumin
 
Creator PATEL, S
SHARMA, KK
DATTA, A
 
Subject VIVO PHOTOSENSITIZING EFFICACY
PHOTODYNAMIC THERAPY
LOW PH
FLUORESCENCE
STATE
SURFACTANTS
DERIVATIVES
COMPONENTS
CURCUMIN
AFFINITY
Chlorin
Specific binding
Sudlow's site
Stopped-flow
Time resolved fluorescence
 
Description The binding of chlorin p(6), a model photosensitizer for photodynamic therapy (PDT), to the Sudlow's site II of Human Serum Albumin (HSA) has been monitored by different spectroscopic methods. Displacement of Dansyl-L-Proline (DP) from its conjugate with HSA is manifested in the spectral shift and decrease in its fluorescence intensity as well as the emergence of component with lifetime of 2-3 ns, which is characteristic of free DP. As DP is known to bind specifically to the Sudlow's site II of human serum albumin, its displacement by chlorin p(6) indicates the residence of the photosensitizer in the same site, in addition to Sudlow's site I. The binding constants for Sudlow's site II, determined by the stopped-flow technique, are found to be two orders of magnitude smaller than that for Sudlow's site I. (C) 2014 Elsevier B.V. All rights reserved.
 
Publisher PERGAMON-ELSEVIER SCIENCE LTD
 
Date 2016-01-15T05:05:55Z
2016-01-15T05:05:55Z
2015
 
Type Article
 
Identifier SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 138(A)925-931
1386-1425
http://dx.doi.org/10.1016/j.saa.2014.10.015
http://dspace.library.iitb.ac.in/jspui/handle/100/17831
 
Language en