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Excited State Dynamics of Brightly Fluorescent Second Generation Epicocconone Analogues

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Title Excited State Dynamics of Brightly Fluorescent Second Generation Epicocconone Analogues
 
Creator CHATTERJEE, S
KARUSO, P
BOULANGE, A
FRANCK, X
DATTA, A
 
Subject ULTRAFAST DYNAMICS
SENSITIVE DETECTION
2-DIMENSIONAL GELS
CHARGE-TRANSFER
BASIS-SETS
PROTEIN
STAIN
DESIGN
FLUOROPHORES
ASSAY
 
Description The natural product epicocconone, owing to its unique fluorescence properties, has been developed into a range of products used in biotechnology, especially proteomics. However, its weak green fluorescence in its native state, while advantageous for proteomics applications, is a disadvantage in other applications that require two-color readouts. Here we report the photophysical characterization of two brightly fluorescent analogues of epicocconone. These analogues, with naphthyl or pyridyl groups replacing the heptatriene chain, resulted in bright fluorescence in both the native state and the long Stokes shifted enamine. Time-resolved fluorescence studies and DFT calculations were carried out to understand the excited state processes involved in fluorescence. Results showed the p-chloro group on the pyridyl is responsible for the high fluorescence of the native fluorophore. The application of one of these compounds for staining electrophoresis gels is exemplified.
 
Publisher AMER CHEMICAL SOC
 
Date 2016-01-15T06:11:21Z
2016-01-15T06:11:21Z
2015
 
Type Article
 
Identifier JOURNAL OF PHYSICAL CHEMISTRY B, 119(20)6295-6303
1520-6106
http://dx.doi.org/10.1021/acs.jpcb.5b02190
http://dspace.library.iitb.ac.in/jspui/handle/100/17899
 
Language en