Record Details

Dynamic association of PfEMP1 and KAHRP in knobs mediates cytoadherence during Plasmodium invasion

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Dynamic association of PfEMP1 and KAHRP in knobs mediates cytoadherence during Plasmodium invasion
 
Creator GANGULY, AK
RANJAN, P
KUMAR, A
BHAVESH, NS
 
Subject HISTIDINE-RICH PROTEIN
ERYTHROCYTE-MEMBRANE PROTEIN-1
FALCIPARUM-INFECTED ERYTHROCYTES
INTRINSICALLY DISORDERED PROTEINS
NMR STRUCTURE DETERMINATION
TITRATION CALORIMETRY DATA
STRUCTURAL BASIS
CHEMICAL-SHIFTS
GLOBAL ANALYSIS
ALPHA-HELIX
 
Description Plasmodium falciparum infected erythrocytes display membrane knobs that are essential for their adherence to vascular endothelia and for prevention of clearance by the spleen. The knob associated histidine rich protein (KAHRP) is indispensable to knob formation and has been implicated in the recruitment and tethering of P. falciparum erythrocyte membrane protein-1 (PfEMP1) by binding to its cytoplasmic domain termed VARC. However, the precise mechanism of interaction between KAHRP and VARC is not very well understood. Here we report that both the proteins co-localize to membrane knobs of P. falciparum infected erythrocytes and have identified four positively charged linear sequence motifs of high intrinsic mobility on KAHRP that interact electrostatically with VARC in solution to form a fuzzy complex. The current study provides molecular insight into interaction between KAHRP and VARC in solution that takes place at membrane knobs.
 
Publisher NATURE PUBLISHING GROUP
 
Date 2016-01-15T06:37:19Z
2016-01-15T06:37:19Z
2015
 
Type Article
 
Identifier SCIENTIFIC REPORTS, 5
2045-2322
http://dx.doi.org/10.1038/srep08617
http://dspace.library.iitb.ac.in/jspui/handle/100/17950
 
Language en