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Fluorescent ZnO for imaging and induction of DNA fragmentation and ROS-mediated apoptosis in cancer cells

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Title Fluorescent ZnO for imaging and induction of DNA fragmentation and ROS-mediated apoptosis in cancer cells
 
Creator GUPTA, J
BHARGAVA, P
BAHADUR, D
 
Subject ZINC-OXIDE NANOPARTICLES
MITOCHONDRIAL DAMAGE
SILICA NANOPARTICLES
OXIDATIVE STRESS
IN-VIVO
TOXICITY
CYTOTOXICITY
DISSOLUTION
NANOCOMPOSITES
ARCHITECTURES
 
Description Fluorescein isothiocyanate (FITC)-encapsulated ZnO nanocomposite has been synthesized using the soft chemical approach. X-ray diffraction reveals the formation of highly crystalline single-phase hexagonal wurtzite nanostructure. TEM and SEM micrographs indicate the formation of spherical porous nanoassembly of ZnO of size similar to 100-400 nm. On the other hand, FITC-ZnO nanocomposite is spherical and porous but with a uniform size of similar to 150 nm. The size of single particles is similar to 20 nm for the ZnO nanoassembly and similar to 15 nm for FITC-ZnO nanocomposite. The UV-visible, fluorescence, FTIR and XPS spectra confirm the formation of FITC-ZnO nanocomposite. The FITC-ZnO nanocomposite demonstrates excellent selectivity in preferential killing of cervical (HeLa) and breast (MCF-7) cancer cells with minimal toxicity to normal fibroblast cells (L929). Apoptotic cells are observed and analyzed by confocal microscopy and flow cytometry. Our results show that cytotoxicity of FITC-ZnO nanocomposite towards cancer cells is due to the generation of reactive oxygen species (ROS) and preferential dissolution of Zn2+ ions in an acidic cancer microenvironment. Furthermore, generated ROS and dissolved Zn+2 ions induce cellular apoptosis, DNA fragmentation, and depolarization of mitochondrial membrane and cell cycle arrest in S phase. The FITC encapsulated multifunctional ZnO nanocomposite can be used as smart nanostructures for cell imaging and cancer therapy.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2016-01-15T06:58:42Z
2016-01-15T06:58:42Z
2015
 
Type Article
 
Identifier JOURNAL OF MATERIALS CHEMISTRY B, 3(9)1968-1978
2050-750X
2050-7518
http://dx.doi.org/10.1039/c4tb01661k
http://dspace.library.iitb.ac.in/jspui/handle/100/17992
 
Language en