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A water soluble glucopyranosyl conjugate as a selective and reactive probe for cysteine in a buffer and its application to living cells

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Title A water soluble glucopyranosyl conjugate as a selective and reactive probe for cysteine in a buffer and its application to living cells
 
Creator ARETI, S
TEOTIA, R
RAO, CP
 
Subject ON FLUORESCENT-PROBE
MOLECULAR PROBE
NEURONAL CELLS
BLOOD-PLASMA
CANCER CELLS
THIOL PROBE
LIVE CELLS
IN-VIVO
GLUTATHIONE
HOMOCYSTEINE
 
Description A water soluble and biocompatible glucopyranosyl conjugate ( L) has been synthesized and characterized by various techniques. The L has been employed to recognize Cys selectively among the naturally occurring amino acids in HEPES buffer at physiological pH. A minimum detection limit of 2.5 x 10(-7) M was shown by L for Cys in the buffer at pH = 7.4. The reactivity of L towards biological thiols as demonstrated by emission and absorption is supported by the observed increase in the fluorescence intensity; however, Cys shows a maximum increase owing to its better nucleophilicity. The reactivity of Cys on L is demonstrated by H-1 NMR, ESI MS, emission and absorption spectroscopy, and the formation of the binary complex was supported by ESI MS. The control molecular study revealed the necessity of the glycomoiety to bring water solubility and biological adaptability. The cellular studies support that the conjugate L is biologically adaptable and shows effective intracellular fluorescence emission upon reacting with intracellular thiols.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2016-01-15T06:59:43Z
2016-01-15T06:59:43Z
2015
 
Type Article
 
Identifier ANALYST, 140(21)7391-7398
0003-2654
1364-5528
http://dx.doi.org/10.1039/c5an01626f
http://dspace.library.iitb.ac.in/jspui/handle/100/17994
 
Language en