Record Details

Comprehensive Analysis of Temporal Alterations in Cellular Proteome of Bacillus subtilis under Curcumin Treatment

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Comprehensive Analysis of Temporal Alterations in Cellular Proteome of Bacillus subtilis under Curcumin Treatment
 
Creator REDDY, PJ
SINHA, S
RAY, S
SATHE, GJ
CHATTERJEE, A
PRASAD, TSK
DHALI, S
SRIKANTH, R
PANDA, D
SRIVASTAVA, S
 
Subject CLP-DEPENDENT PROTEOLYSIS
LARGE GENE LISTS
ESCHERICHIA-COLI
DIVISION
MEMBRANE
SPORULATION
PROTEINS
BACTERIA
FTSZ
BIOSYNTHESIS
 
Description Curcumin is a natural dietary compound with antimicrobial activity against various gram positive and negative bacteria. This study aims to investigate the proteome level alterations in Bacillus subtilis due to curcumin treatment and identification of its molecular/cellular targets to understand the mechanism of action. We have performed a comprehensive proteomic analysis of B. subtilis AH75 strain at different time intervals of curcumin treatment (20, 60 and 120 min after the drug exposure, three replicates) to compare the protein expression profiles using two complementary quantitative proteomic techniques, 2D-DIGE and iTRAQ. To the best of our knowledge, this is the first comprehensive longitudinal investigation describing the effect of curcumin treatment on B. subtilis proteome. The proteomics analysis revealed several interesting targets such UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1, putative septation protein SpoVG and ATP-dependent Clp protease proteolytic subunit. Further, in silico pathway analysis using DAVID and KOBAS has revealed modulation of pathways related to the fatty acid metabolism and cell wall synthesis, which are crucial for cell viability. Our findings revealed that curcumin treatment lead to inhibition of the cell wall and fatty acid synthesis in addition to differential expression of many crucial proteins involved in modulation of bacterial metabolism. Findings obtained from proteomics analysis were further validated using 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) assay for respiratory activity, resazurin assay for metabolic activity and membrane integrity assay by potassium and inorganic phosphate leakage measurement. The gene expression analysis of selected cell wall biosynthesis enzymes has strengthened the proteomics findings and indicated the major effect of curcumin on cell division.
 
Publisher PUBLIC LIBRARY SCIENCE
 
Date 2016-01-15T10:13:15Z
2016-01-15T10:13:15Z
2015
 
Type Article
 
Identifier PLOS ONE, 10(4)
1932-6203
http://dx.doi.org/10.1371/journal.pone.0120620
http://dspace.library.iitb.ac.in/jspui/handle/100/18341
 
Language en