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Coupling of Cellular Processes and Their Coordinated Oscillations under Continuous Light in Cyanothece sp ATCC 51142, a Diazotrophic Unicellular Cyanobacterium

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Title Coupling of Cellular Processes and Their Coordinated Oscillations under Continuous Light in Cyanothece sp ATCC 51142, a Diazotrophic Unicellular Cyanobacterium
 
Creator KRISHNAKUMAR, S
GAUDANA, SB
VINH, NX
VISWANATHAN, GA
CHETTY, M
WANGIKAR, PP
 
Subject NITROGEN-FIXING CYANOBACTERIA
CIRCADIAN GENE-EXPRESSION
SYNECHOCOCCUS-ELONGATUS
RNA HELICASE
MESSENGER-RNA
SIGMA-FACTORS
CLOCK
RHYTHM
IDENTIFICATION
METABOLISM
 
Description Unicellular diazotrophic cyanobacteria such as Cyanothece sp. ATCC 51142 (henceforth Cyanothece), temporally separate the oxygen sensitive nitrogen fixation from oxygen evolving photosynthesis not only under diurnal cycles (LD) but also in continuous light (LL). However, recent reports demonstrate that the oscillations in LL occur with a shorter cycle time of similar to 11 h. We find that indeed, majority of the genes oscillate in LL with this cycle time. Genes that are upregulated at a particular time of day under diurnal cycle also get upregulated at an equivalent metabolic phase under LL suggesting tight coupling of various cellular events with each other and with the cell's metabolic status. A number of metabolic processes get upregulated in a coordinated fashion during the respiratory phase under LL including glycogen degradation, glycolysis, oxidative pentose phosphate pathway, and tricarboxylic acid cycle. These precede nitrogen fixation apparently to ensure sufficient energy and anoxic environment needed for the nitrogenase enzyme. Photosynthetic phase sees upregulation of photosystem II, carbonate transport, carbon concentrating mechanism, RuBisCO, glycogen synthesis and light harvesting antenna pigment biosynthesis. In Synechococcus elongates PCC 7942, a non-nitrogen fixing cyanobacteria, expression of a relatively smaller fraction of genes oscillates under LL condition with the major periodicity being 24 h. In contrast, the entire cellular machinery of Cyanothece orchestrates coordinated oscillation in anticipation of the ensuing metabolic phase in both LD and LL. These results may have important implications in understanding the timing of various cellular events and in engineering cyanobacteria for biofuel production.
 
Publisher PUBLIC LIBRARY SCIENCE
 
Date 2016-01-15T10:13:45Z
2016-01-15T10:13:45Z
2015
 
Type Article
 
Identifier PLOS ONE, 10(5)
1932-6203
http://dx.doi.org/10.1371/journal.pone.0125148
http://dspace.library.iitb.ac.in/jspui/handle/100/18342
 
Language en