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Isolation and characterization of antagonistic Streptomyces spp. from marine sediments along the southwest coast of India

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Relation http://eprints.cmfri.org.in/10805/
http://nopr.niscair.res.in/handle/123456789/3
 
Title Isolation and characterization of antagonistic Streptomyces spp. from
marine sediments along the southwest coast of India
 
Creator Chakraborty, Rekha D
Chakraborty, Kajal
Thilakan, Bini
 
Subject Biochemistry
 
Description Antagonistic Streptomyces spp. were isolated from marine and mangrove sediment samples collected off Cochin,
along the southwest coast of India. Sediment samples were pre-treated and following the soil dilution technique, samples were
surface plated on starch casein agar and actinomycetes isolation agar. In the primary screening, 7.4% of presumptive
actinomycetes (135 isolates) showed antibacterial activity against one or more bacterial fish pathogens and 3.7% of these
cultures showed broad spectrum activity against the tested pathogens. Morphologically white powdery colonies with chalky
white /grey appearance were selected as presumptive Streptomyces cultures. Isolates subjected to biochemical, physiological and
16S rDNA characters revealed the presence of three species of Streptomyces dominated by Streptomyces tanashiensis followed
by S. viridobrunneus and S. bacillaris. Isolates characterized by 16S rDNA indicated the presence of 650 bp band in
Streptomyces spp. Primary screening for activity against selected fish pathogens was done by a cross streak method using
modified nutrient agar medium. Prominent isolates showing high zone of activity against the fish pathogens ranged 17-35 mm by
the paper disc method. Enriched broth of selected isolates showing high antagonistic activity was screened for pharmacologically
active agents revealed ethyl acetate fractions to be active against selected microbial pathogens.
 
Date 2015
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/10805/1/Indian%20Journal%20of%20Geomarine%20Sciences_2015_44_1_Isolation%20and%20characterization_Kajal.pdf
Chakraborty, Rekha D and Chakraborty, Kajal and Thilakan, Bini (2015) Isolation and characterization of antagonistic Streptomyces spp. from marine sediments along the southwest coast of India. Indian Journal of Geo-Marine Sciences, 44 (1).