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DNA barcoding of gobiid fishes (Perciformes, Gobioidei)

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Relation http://eprints.cmfri.org.in/10866/
 
Title DNA barcoding of gobiid fishes (Perciformes, Gobioidei)
 
Creator Viswambharan, Divya
Pavan Kumar, Annam
Singh, Dhirendra P
Jaiswar, A K
Chakraborty, S K
Nair, J Rajasekharan
Lakra, W S
 
Subject Fish Genetics
 
Description Gobiids constitute a major proportion of fish population in both tropical and temperate
freshwater as well as marine ecosystem. Due to their small size, cryptic ecology and ambiguous
morphological characters, gobiids diversity was not documented completely. In this study, DNA
barcodes were generated for 11 species of gobiids, collected from the Ashtamudi Lake, India.
The mitochondrial COI gene was amplified using universal primers and the resulted 650 bp
amplicon was sequenced. The COI barcodes clearly distinguished all the species with high interspecific
genetic distance values than intra-specific values based on K2P (Kimura 2 Parameter)
model. The average genetic distance (K2P model) within species, genus and family was 1.2%,
22.2% and 25.3%, respectively. In addition to barcode-based species identification system,
Nucleotide Diagnostic (ND) characters specific for species were identified. The Neighbor-Joining
tree revealed distinct clusters shared by the species of same genera.
 
Date 2015
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/10866/1/DNA%20barcoding%20of%20gobiid%20fishes_Mitochondrial%20DNA_2015_26-1-15-19.pdf
Viswambharan, Divya and Pavan Kumar, Annam and Singh, Dhirendra P and Jaiswar, A K and Chakraborty, S K and Nair, J Rajasekharan and Lakra, W S (2015) DNA barcoding of gobiid fishes (Perciformes, Gobioidei). Mitochondrial DNA, 26 (1). pp. 15-19.