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Antibody studies and bacterial drug resistance in typhoid fever

Shodhganga@INFLIBNET

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Title Antibody studies and bacterial drug resistance in typhoid fever
 
Contributor Harish, B N
 
Description Typhoid fever is a major cause of mohidity and mortality in the developing
world. The causative organism, Salmonella Typhi, has developed resistance to many of
the antibiotics used in freatment. It is essential to monitor the development of antibiotic
resistance in order to formulate effective treatment policies. Typhoid fever is also
associated with life threatening complications like ileal perforations, the pathogenesis of
which is poorly understood.
Aim and Objectives: The aims of this study were twofold-I) To look at antibiotic
resistance in S. Typhi in general and fluoroquinolonc resistance in particular, and 2) To
investigate the antibody profile in typhoid perforation.
Materials and Methods This study included isolates of S.Typhi and serum samples
from palicnts with signs and symptoms of typhoid fever, Isolation and identification
followed standard pro~ocols. Antibiotic susceptibility was determined by Kirby- Bauer
disc diffision method for a panel of antibiotics. The MIC was determined by both agar
dilution and broth microdilution for ampicillin, chloramphenicol, ciprofloxacin and
ceftriaxone and interpreted in accordance with NCCLS guidelines. Envelope profiling
and PCR-RFLP were done for 30 selected isolates. PCR w a designed and performed for
the amplification of 4 sequences located in the genes gyrA, morA, marl?, and rnarR.
For antibody studies, 3 groups of samples were analysed- Group I, from patients
with typhoid iled perforations. Group 11, from patients with uncomplicated typhoid fever
md Gmup 111, consisting of healthy individuals or patienb with non-enteric infections.
Antibodies were detected in these sera by immunoblotting using Whole Cell Rotein
(WCP) extract, and also individually lor the H, LPS and OMP antigens.
Results and d i d o n : During the 35 months of this study, 177 isolates of S.Typhi
were obtained. Multidmg resistance was seen to decline over this period from 568 to
7%. In conhst to multidrug resistance, reduced ciprofloxacin susceptibility was seen in a
majority of the isolates- 159, of which 157 were also nalidixic acid resistant. There was a
good cornlation hetween nalidixic acid resistance and reduced ciprofloxacin
susceptibility.
References xlixp.
 
Date 2010-11-03T09:42:01Z
2010-11-03T09:42:01Z
2010-11-03
May 2006
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/946
T 3747
 
Language English
 
Rights university
 
Format 258p.
CD
 
Publisher Pondicherry
Pondicherry University
Jawaharlal Institute of Postgraduate Medical Educarion and Research.Department of Microbiology
 
Source INFLIBNET