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Rapid identification of bacterial pathogens causing subclinical bovine mastitis with special reference to Staphylococcus aureus Escherichia coli and predominant Streptococcal species by molecular methods

Shodhganga@INFLIBNET

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Title Rapid identification of bacterial pathogens causing subclinical bovine mastitis with special reference to Staphylococcus aureus Escherichia coli and predominant Streptococcal species by molecular methods
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Contributor Isloor, Shrikrishna
 
Subject Veterinary Sciences
Veterinary Microbiology
Ssubclinical mastitis
 
Description The present study was carried out with objectives to screen milk samples for
newlinesubclinical mastitis (SCM) by employing somatic cell count and electrical conductivity tests, to isolate and identify major bacterial pathogens Staphylococcus aureus, Escherichia coli and predominant Streptococcal species from SCM cases and to
newlinestandardize simplex and multiplex PCR for rapid detection of these pathogens in milk
newlinesamples. Out of 246 milk samples screened for SCM, 186 milk samples were subjected for isolation and 85 Streptococci, 95 S. aureus, 95 CoNS and 48 E. coli isolates were
newlineobtained. Polymerase chain reaction was standardized targeting tuf gene to identify
newlineStreptococci and Staphylococci at genus level, 16S rRNA gene to identify S. agalactiae, S. dysgalactiae, S. uberis and further, sip and pauA gene to identify S. agalactiae and S. uberis respectively. The screening of 85 Streptococcal isolates revealed seven isolates as S. agalactiae. Staphylococcus aureus was identified by nuc and sodA gene based PCR. Screening of 95 S. aureus isolates revealed the presence of nuc gene in all isolates and sodA gene in 87 isolates. Forty eight isolates of E. coli were screened and confirmed by alr gene based simplex and by a multiplex PCR (m-PCR). A two tube m-PCR was standardized to simultaneously detect the five major mastitis pathogens from milk samples. Screening of 147 bulk milk samples detected major pathogens in 81 bulk milk samples. Staphylococcus aureus was the predominant pathogen detected, followed by E.
newlinecoli, S. dysgalactiae and S. uberis. The m-PCR assay developed in the present study was
newlinean easy and rapid method to simultaneously detect the five major mastitis pathogens in
newlinebulk milk. The regular analysis of bulk milk by m-PCR developed in the study may
newlinebecome an useful tool for determining the herd status in the detection of contagious and environmental mastitis pathogens.
Bibliography p. 168-192
 
Date 2013-02-19T06:43:17Z
2013-02-19T06:43:17Z
2013-02-19
n.d.
27/05/2011
2011
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/6953
 
Language English US
 
Relation --
 
Rights university
 
Format 193p.
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None
 
Coverage Veterinary Microbiology
 
Publisher Bidar
Karnataka Veterinary, Animal and Fisheries Sciences University
Department of Veterinary Microbiology
 
Source INFLIBNET