Record Details

Anticarcinogenic Activity of Stress Induced Lactobacillus Acidophilus MTCC 447

Shodhganga@INFLIBNET

View Archive Info
 
 
Field Value
 
Title Anticarcinogenic Activity of Stress Induced Lactobacillus Acidophilus MTCC 447
—
 
Contributor Winkins Santosh.S
 
Subject —
 
Description In the beginning of the 20th century, the Russian Nobel prize winner Elie
newlineMetchnikoff observed high life expectancy in Bulgarian persons who ate large
newlineamounts of fermented milk products. Based on this observation the followed research
newlineemphasized, that Lactic acid bacteria plays an important role in retarding colon
newlinecarcinogenesis by possibly influencing metabolic, immunologic and protective
newlinefunctions in the colon. Various studies have indicated that L. acidophilus MTCC447
newlinemay alleviate retarding colon carcinogen, but ratio of carcinogen neutralisation was
newlinediffer for lab condition (in vitro) and intestinal ecosystem (in vivo) is 1: 5 respectively,
newlinethe mechanism of intracellular neutralization of carcinogen is also unknown. The aim
newlineof the study was (1) To study the anti-carcinogenicity activity of L. acidophilus
newlineMTCC 447 and L. acidophilus M, (2) To study the role of abiotic stress (related to
newlinehuman intestine) such as Temperature (35 to 60ยบ C) pH (2 to 9), salinity (0.1 to 0.8m
newlineNaCl) on carcinogenic protection activity of L. acidophilus MTCC 447 and
newlineL. acidophilus M, (3) To study the role of plasmid on anti-carcinogenic activity in
newlinestress environment, (4) To study role of stress protein from L. acidophilus MTCC447
newlineon anticarcinogenic activity. L. acidophillus MTCC447 were assayed to determine
newlinetheir potential effects on two reference genotoxins: the 4-NQO, which produces DNA
newlineadducts and the MNNG, a DNA alkylation agent. Antigenotoxicity was studied both
newlinein prokaryotic and eukaryotic cells, using the SOS-Chromotest, a primary DNA
newlinedamage assay on Escherichia coli and the Comet assay, for analyzing DNA breakage
newlinein enterocytes.
newline
—
 
Date 2014-12-19T09:49:17Z
2014-12-19T09:49:17Z
2014-12-19
13-3-2007
—
—
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/31191
 
Language English
 
Relation —
 
Rights university
 
Format —
—
DVD
 
Coverage —
 
Publisher Kattankulathur
SRM University
Department of Biotechnology
 
Source University