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Studies on Callus Induction Cell Suspension Culture and Characterization of Saponin from Memecylon Edule Roxb

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Title Studies on Callus Induction Cell Suspension Culture and Characterization of Saponin from Memecylon Edule Roxb
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Contributor Kantha D. Arunachalam
 
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Description newline Saponins are important bioactive steroidal compounds utilized in the
newlinepharmaceutical industries. They are found in many plant species and have a wide
newlinerange of biological properties which makes them an interesting research subject.
newlineThe small shrubs or tree Memecylon edule is used in traditional medicine against
newlinevarious diseases. The first part of the present research study was aimed at the
newlineproduction and enhancement of saponin by means of in vitro culture; isolation of
newlinesaponin from M.edule leaves in vivo. The other part of this study covered two
newlineaspects. The first aspect related to results of experiments on the possible
newlinepharmacological uses of M.edule saponin from haemolytic, pisicidal, insecticidal,
newlineanticancer, antioxidant and antimicrobial angles. The second aspect touched a new
newlinefield namely that M.edule extract is a good source for the green synthesis of gold
newlineand silver nanoparticles. A detailed narrative follows.
newlineThe aim of the study was focused on in vitro culture of Memecylon edule
newlinefor producing saponin and to study the cell growth rate. Leaf and nodal segments of
newlineMemecylon edule were cultured on half and full strength Murashige and skoog
newline(MS) medium and Woody plant medium (WPM) which also contained different
newlineconcentrations of growth regulators and antioxidants. For the first time, this study
newlinehas standardized the medium and hormone combination to initiate callus from
newlineM.edule. The green and brown friable callus and brown compact callus were
newlineinduced from nodal segments and white compact callus was induced from leaf of
newlineM.edule.
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Date 2014-12-19T09:50:35Z
2014-12-19T09:50:35Z
2014-12-19
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Type Ph.D.
 
Identifier http://hdl.handle.net/10603/31195
 
Language English
 
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Rights university
 
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DVD
 
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Publisher Kattankulathur
SRM University
Department of Biotechnology
 
Source University