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Optimization of Laccase Enzy me Production Through Submerged and Solid State Fermentation of Agro Wastes By the Fungi Isolated from Saw Mill Wastes and Its Application in the Biodegradation of Lignin Cypermethrin and Biodecolourization of Azo Dyes

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Title Optimization of Laccase Enzy me Production Through Submerged and Solid State Fermentation of Agro Wastes By the Fungi Isolated from Saw Mill Wastes and Its Application in the Biodegradation of Lignin Cypermethrin and Biodecolourization of Azo Dyes
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Contributor Kantha D.Arunachalam
 
Subject —
 
Description Sixteen fungal samples, collected from saw mill wastes, were screened for
newlinetheir laccase producing ability. Five isolates were selected, based on their quantity
newlineof laccase enzyme production, for the study. Trametes versicolor was found to be
newlinethe suitable fungi, among the isolates, in terms of laccase enzyme production.
newlineSemi-synthetic media and agro waste based media were screened for their support
newlinefor laccase enzyme production and it was found that sapota seed based media
newlinesupported the maximum laccase enzyme production (2.34 U/ml). The media
newlineoptimization was carried out by classical and statistical methods. In classical
newlinemethod, the effect of various carbon and nitrogen sources along with the effect of
newlinetemperature, pH and shaking speed were studied and it was found that glucose
newlinewas the best carbon source, peptone best nitrogen source, 30°C optimum
newlinetemperature, 6.0 as the optimum pH and 150 rpm as the best shaking speed for
newlinemaximum laccase enzyme production viz., 2.61 U/ml. Vermi wash was used as a
newlinenutritional supplement in the culture media and it was found that 50%
newlineconcentration of vermi wash supported the good growth of fungi and laccase
newlineenzyme production (2.74 U/ml).
newline
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Date 2014-12-30T05:04:06Z
2014-12-30T05:04:06Z
2014-12-30
11-4-2007
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Type Ph.D.
 
Identifier http://hdl.handle.net/10603/32048
 
Language English
 
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Rights university
 
Format —
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DVD
 
Coverage —
 
Publisher Kattankulathur
SRM University
Department of Biotechnology
 
Source University