ICAR Research Data Repository for Knowledge Management
Development of phage display systems for functional genomics
Shodhganga@INFLIBNET
View Archive Info| Field | Value | |
| Title |
Development of phage display systems for functional genomics
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| Contributor |
Chaudhary, Vijay K
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| Subject |
Biochemistry
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| Description |
The phage display is a powerful technique for studying protein-ligand interactions. Since efficient identification of protein physical association is expected to have major impact on the elucidation of protein functions, phage display can prove to newlinebe a prime technology for Functional Genomics . This will be greatly facilitated if newlinephage display library of genome-wide proteome is available. In phage display, the newlineDNA encoding a peptide or protein is cloned in frame with a coat protein of a newlinebacteriophage, which is then displayed as fusion protein on the phage surface while the newlineencoding DNA is encapsulated in the same phage, thus providing the direct link newlinebetween the phenotype (the displayed protein) and the genotype (the encoding DNA). newlineBecause of this unique property, a phage displaying a peptide or protein can be newlineenriched from a milieu of millions of other phages by process of panning (affinity newlineselection). Several bacteriophages like Lambda, T7, T4 have been employed for newlinedisplaying proteins. Generally, gIIIp of M13 filamentous phage has been used for Nterminal newlinedisplay where the DNA is cloned in frame between the signal sequence and newlinethe coding sequence of gIIIp. But, in the case of gIIIp display, only 1 out of 18 insert newlinedisplay functional protein (genic protein; matching with the proteome of the organism). newlineThe work embodied in this thesis describes designing of a system for high newlineefficiency directional cloning of randomly fragmented genome of M. tuberculosis in a newlinegIIIp based phagemid display vector between PelB signal sequence (PelBss) and fulllength newlinegIIIp (aa 2 - 406) using a restriction enzyme free cloning method developed in newlineour laboratory. Bibliography p.175-192, Appendix p.193-234 |
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| Date |
2014-04-28T10:53:28Z
2014-04-28T10:53:28Z 2014-04-28 n.d. 2012 n.d. |
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| Type |
Ph.D.
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| Identifier |
http://hdl.handle.net/10603/18005
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| Language |
English US
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| Relation |
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| Rights |
university
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| Format |
234p.
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| Coverage |
Biochemistry
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| Publisher |
New Delhi
University of Delhi Dept. of Biochemistry |
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| Source |
INFLIBNET
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