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Characterization of midgut specific gene in Indian malaria vector An. culicifacies (Diptera: Culicidae)

Shodhganga@INFLIBNET

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Title Characterization of midgut specific gene in Indian malaria vector An. culicifacies (Diptera: Culicidae)
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Contributor Gakhar, S K
Dash, A P
 
Subject Bio Technology
Indian malaria vector
Diptera
 
Description The present investigations have been carried out on Indian malaria vector Anopheles culicifacies, which is responsible for about 65-70% of malaria in India. During this study an attempt has been made to develop the indigenous techniques for mosquito transgenesis and to identify an effector/ antiparasitic molecule for transmission blocking and for anti-mosquito immunity. For this, the ultra structure of An. culicifacies eggs has been observed with the help of scanning electron microscopy so as to prepare the eggs for microinjection/ transgenesis. An. culicifacies egg were boat shaped, black in color, moderate float length (14 15ridges) and 3 oval shaped lobed tubercles at both anterior and posterior ends. Micropylar orifice was surrounded by collar with incomplete hexagonal rays. Chorionic cells were present on anterior-lateral surface with distinct boundries. The frill was moderate in height. The ultra structural analysis should enable to prepare the eggs for microinjection. In addition, the construction of pBac [3xP3-EGFP-Afm] plasmid was standarized for microinjection. The temporal pattern of soluble proteins in the midgut of An. culicifacies has been studied to reveal the polypeptides involved in glucose feeding. The age and sex-specific polypeptides in the midgut have also been investigated. The soluble midgut proteins pattern of different species of Anopheles and in sibling species complex of An. Culicifacies (Type A, B and C) has also been investigated. N-terminal sequencing of low molecular weight polypeptides was also analyzed. N-terminal sequencing of low molecular weight proteins and sequence analysis shows that 13 kDa polypeptide was ~80% homologous to An. gambiae cecropin like poltpeptide. Hence, 13 kDa polypeptide was used to raise antisera. The antibody titer was measured by ELISA. The putative immunogenic polypeptides were identified by Western blotting. The binding of antibodies with different tissues was studied by in vivo ELISA.
Bibliography p.122-148, Appendix p.149-152
 
Date 2013-04-01T11:06:04Z
2013-04-01T11:06:04Z
2013-04-01
n.d.
2011
n.d.
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/7833
 
Language English
 
Relation -
 
Rights university
 
Format 152p.
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None
 
Coverage Bio Technology
 
Publisher Rohtak
Maharshi Dayanand University
Department of Bio Technology
 
Source INFLIBNET