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Structure-function relationship in escherichia coli RNA polymerase subunits : a case study with a-subunit

Shodhganga@INFLIBNET

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Title Structure-function relationship in escherichia coli RNA polymerase subunits : a case study with a-subunit
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Contributor Chatterjee, Dipankar
 
Subject Molecular Biology
Cellular Biology
polymerase
RNA
 
Description Specific protein-protein and protein-DNA interactions govern the regulation of all cellular
newlineprocesses which constitute a living cell. The basis of all essential biological functions is the
newlineability of the cell to elicit the required cellular response by specific molecular interactions.
newlineFor example, replication apparatus, transcription machinery, translation complexes,
newlineenzyme assemblies, receptor-ligand interactions and transport pathways all mediate their
newlinerespective functions by cross-talk between specific macromolecules. Protein-protein
newlineinteraction is an important subset of a variety of interactions constituting biological
newlineprocesses.
newlineThe transcription apparatus in Escherichia coli consists of a DNA dependent RNA
newlinepolymerase (RNAP) which is a multi-subunit enzyme with subunit composition of
newlinea 2j3W (l)(J. Interactions within and between the subunits result in the formation of
newlinecatalytically competent enzyme capable of .promoter specific initiation of transcription.
newlineMoreover, specific interactions between RNAP and transcriptions activators or DNA fine
newlinetune the transcription apparatus to meet the myriad cellular needs. Thus, an insight into
newlineprotein-protein interactions provides a basis for understanding the highly ordered and
newlineregulated process of transcription in E.coli.
newlineChapter I outlines the various in vitro biochemical methods and also in particular,
newlinein vivo genetic approach employed to study protein-protein interactions. Genetic
newlineapproaches encompassing the various mechanisms of suppression is discussed. This
newlinechapter also outlines the power of genetic suppression as a tool to probe protein-protein
newlineinteractions citing recent examples, specifically in understanding structure-function
newlinerelationship within multisubunit complexes, followed by a brief description of E.coli RNA
newlinepolymerase and its subunits.
newlineChapter II describes a method to detect Zn (II) binding proteins and the results with
newlineE.coli RNA polymerase which is a Zn (II) metalloenzyme.
References given, Abbrevations p.i, Publications p.vi
 
Date 2014-01-17T09:04:00Z
2014-01-17T09:04:00Z
2014-01-17
n.d.
1999
n.d.
 
Type Ph.D.
 
Identifier http://hdl.handle.net/10603/15162
 
Language English
 
Relation -
 
Rights university
 
Format iv, 54p.
-
None
 
Coverage Biology
 
Publisher Delhi
Jawaharlal Nehru University
Centre for Molecular and Cellular Biology
 
Source INFLIBNET