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Nuclear and mitochondrial DNA markers based identification of blunthorn lobster Palinustus waguensis Kubo, 1963 from South-west coast of India

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Relation http://eprints.cmfri.org.in/11313/
 
Title Nuclear and mitochondrial DNA markers based identification of blunthorn lobster

Palinustus waguensis Kubo, 1963 from South-west coast of India
 
Creator Chakraborty, Rekha D
Maheswarudu, G
Purushothaman, P
Kuberan, G
Sebastian, Jomon
Radhakrishnan, E V
Thangaraja, R
 
Subject Fish Biotechnology
Lobsters
Fish Genetics
 
Description The present study reports the occurrence of Palinustus waguensis Kubo, 1963 from South-west coast of India on the basis

of morphological as well as molecular data. The DNA sequences of two mitochondrial genes, large subunit ribosomal DNA

(16S rDNA) and cytochrome oxidase I (COI), and two nuclear-protein coding genes, viz., phosphoenolpyruvate

carboxykinase (PEPCK) and sodium-potassium ATPase α-subunit (NaK), were submitted in the GenBank. Morphological

examination of the specimen confirmed the identity of the species as P. waguensis. The pairwise genetic distance analysis,

sequence identity and phylogenetic tree have shown that the specimen has similarity only with 16S rDNA sequence of

P. waguensis of earlier reports. In the case of other genetic markers, it has shown some identity towards P. unicornutus and

P. holthuisi, but with a high genetic difference. This study with sequence data of P. waguensis in GenBank will help to

identify the specimen on molecular basis in future.
 
Date 2016
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11313/1/IJBT%2015%282%29%20172-177%20Palinustus%20waguiensis_Rekha%20Chakraborty.pdf
Chakraborty, Rekha D and Maheswarudu, G and Purushothaman, P and Kuberan, G and Sebastian, Jomon and Radhakrishnan, E V and Thangaraja, R (2016) Nuclear and mitochondrial DNA markers based identification of blunthorn lobster Palinustus waguensis Kubo, 1963 from South-west coast of India. Indian Journal of Biotechnology, 15. pp. 172-177.