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Studies on sweet potatoes-III. Distribution of unit chains of branched and unbranched molecules of starch

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Relation http://ir.cftri.com/2/
FC-57-2-201-204-1996
 
Title Studies on sweet potatoes-III. Distribution
of unit chains of branched and unbranched
molecules of starch
 
Creator Madhusudhan, B.
Gowda, L. R.
Tharanathan, R. N.
 
Subject 02 Potato
 
Description

Freshly prepared sweet potato (SP) starch was separated quantitatively into amylose and amylopectin by a concanavalin A precipitation method. Amylose was further fractionated into two subfractions containing essentially linear (Am) and moderately branched (Ax) molecules, by hot-butanol extraction. The mole-
cular weight determined by GPC of Am was 3 x 106 Da, the values for Ap and Ax were 1.5x lo7 and 5 x 1O6 Da, respectively. The Beta-amylolysis limit values were 57,
70 and 92%, respectively, for Ap, Ax and Am. The native, as well as the derived Beta-limit dextrins of Ap and Ax, were debranched with isoamylase and their unit -
chain lengths, measured by GPC and SE-HPLC methods, showed Ap (CL, 21.0)to have a trimodal distribution of unit chains with m values of 52, 37 and 20, -whereas Ax had tetramodal unit chains with DP values of 40, 30, 21 and 9. Am
was more or less a linear molecule. In vitro SP starch was more digestible than legume and cereal starches.


 
Date 1996
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Rights
 
Identifier http://ir.cftri.com/2/1/Food_Chemistry_57%282%29_201-204_1996.pdf
Madhusudhan, B. and Gowda, L. R. and Tharanathan, R. N. (1996) Studies on sweet potatoes-III. Distribution of unit chains of branched and unbranched molecules of starch. Food Chemistry, 57 (2). pp. 201-204. ISSN 0308-8146