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Influence Of Exogenous Hormones On Growth And Secondary Metabolite Production In Hairy Root Cultures Of Cichorium Intybus L. Cv. Lucknow Local

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In vitro-01-01
 
Title Influence Of Exogenous Hormones On Growth And Secondary Metabolite Production
In Hairy Root Cultures Of Cichorium Intybus L. Cv. Lucknow Local
 
Creator Bais, H. P.
Sudha, G.
George, J.
Ravishankar, G. A.
 
Subject 03 Mushroom
10 Plants
05 Tissue Culture
 
Description The effect of exogenously fed hormones on hairy root cultures of Cichorium intybus L. cv. Lucknow Local was studied. It
was seen that auxin in the presence of low levels of kinetin induces rapid disorganization in hairy root cultures of C.
intybus, ultimately to form suspension cultures, and this process was associated with the decrease in coumarin content in
the cells. Of various treatments, it was observed that with an increase in the auxin : cytokinin ratio, the biomass decreased
with the increase in disorganization index during the culture period of 28 d. The disorganization index was less when the
inoculum size was enhanced to 10-fold. The total endogenous indole-3-acetic acid titers and indole-3-acetic acid oxidase
activity also decreased with an increase in disorganization index, and was independent of initial inoculum size, with only a
magnitude difference. The total coumarin content strictly correlated with growth in all the treatments. In contrast,
exogenously supplied gibberellic acid at the 0.5 mg l21 level enhanced growth, coumarin content, and branching patterns
over the control and other treatments on day 28. The exogenously fed growth regulators had an effect on growth, auxin and
coumarin biosyntheses, wherein transformed roots treated with increasing concentration of auxin to cytokinin ratios lost
their ability for coumarin biosynthesis. The behavior of hairy roots from an Indian cultivar of chicory upon growth regulator
treatment is discussed in terms of growth, coumarin and auxin biosyntheses.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/14/1/In_Vitro_Cellular_%26_Developmental_Biology_-_Plant__37_293-.pdf
Bais, H. P. and Sudha, G. and George, J. and Ravishankar, G. A. (2001) Influence Of Exogenous Hormones On Growth And Secondary Metabolite Production In Hairy Root Cultures Of Cichorium Intybus L. Cv. Lucknow Local. In Vitro Cellular & Developmental Biology - Plant, 37. pp. 293-299.