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Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit

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Relation http://ir.cftri.com/31/
http://www.elsevier.com/locate/foodchem
FC-87-551-557-2004
 
Title Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit
 
Creator Kulkarni, Anand. P.
Aradhya, S. M.
Divakar, S.
 
Subject 24 Fruits
 
Description

Pith and carpellary membrane (CM) constitute 13% of pomegranate fruit composition. Pith acts as an anchoring tissue while CM acts as a protective membrane; both play significant roles in determining the health and disease status of pomegranate arils
but remain unexplored for their nutraceutical properties. Activity-guided repeated fractionation of the methanol extract on a silica gel column, followed by preparative HPLC, yielded a compound which exhibited strong radical scavenging activity with EC50 values of 16.7 plus or minum 2.3 and 54.2 plus or minus 4.6 against DPPH free radical and superoxide radicals, respectively. It also showed a very strong lipid peroxidation inhibitory activity in a liposome model system with an EC50 value of 54.2 plus or minus 0.9. However, it showed negligible metal chelating activity. Based on UV, IR, 2D-NMR, GC-Mass and MALDI-Mass studies, the compound was identified as punicalagin. The antioxidant activity of punicalagin and the methanol extract can be ascribed mainly to radical scavenging
ability.
 
Date 2004
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/31/1/Food_Chemistry_87_%282004%29_551-557.pdf
Kulkarni, Anand. P. and Aradhya, S. M. and Divakar, S. (2004) Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit. Food Chemistry, 87. pp. 551-557. ISSN 0308-8146