ICAR Research Data Repository for Knowledge Management

An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for evaluation of
chapati-making quality of wheat varieties. Polyclonal antibodies against gliadin, low molecular weight
glutenin (LMG), and high molecular weight glutenin (HMG) were developed and utilized in the ELISA. Twenty-eight Indian wheat varieties were utilized in the ELISA. Out of these antibodies, an antigliadin antibody response was negatively correlated with farinograph water absorption ( r) -0.89 at P less than 0.01), chapati dough water absorption ( r) -0.91 at P less than 0.01), overall chapati sensory score ( r)-0.95 at P less than 0.01), chapati puffed height score ( r) -0.95 at P less than 0.01), and positively correlated with shear value of chapati ( r) 0.76 at P less than 0.01). Anti-LMG antibody response was not correlated with any of these parameters, whereas anti-HMG response positively correlated with chapati dough
water absorption ( r) 0.44 at P less than 0.05), farinograph water absorption ( r) 0.45 at P less than 0.05), and overall chapati sensory score ( r) 0.44 at P less than 0.05), and negatively correlated with shear value ( r)-0.38 at P less than 0.05) and chapati puffed height ( r) -0.44 at P less than 0.05). The results indicate that wheat varieties with good chapati-making quality were having less antigliadin antibody response.