ICAR Research Data Repository for Knowledge Management

Antioxidant-rich fractions were extracted from pomegranate ( Punica granatum) peels and seeds using ethyl acetate, methanol, and water. The extracts were screened for their potential as antioxidants
using various in vitro models, such as ‚-carotene-linoleate and 1,1-diphenyl-2-picryl hydrazyl (DPPH)
model systems. The methanol extract of peels showed 83 and 81% antioxidant activity at 50 ppm using the ‚-carotene-linoleate and DPPH model systems, respectively. Similarly, the methanol extract of seeds showed 22.6 and 23.2% antioxidant activity at 100 ppm using the ‚-carotene-linoleate and DPPH model systems, respectively. As the methanol extract of pomegranate peel showed the highest
antioxidant activity among all of the extracts, it was selected for testing of its effect on lipid peroxidation,
hydroxyl radical scavenging activity, and human low-density lipoprotein (LDL) oxidation. The methanol extract showed 56, 58, and 93.7% inhibition using the thiobarbituric acid method, hydroxyl radical scavenging activity, and LDL oxidation, respectively, at 100 ppm. This is the first report on the antioxidant properties of the extracts from pomegranate peel and seeds. Owing to this property, the
studies can be further extended to exploit them for their possible application for the preservation of food products as well as their use as health supplements and neutraceuticals.