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Structural Studies of the Proteinase Inhibitors of Germinated Horsegram(Dolichos Biflorus) Seeds

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Title Structural Studies of the Proteinase Inhibitors of Germinated Horsegram(Dolichos Biflorus) Seeds
 
Creator Pradeep, Kumar
 
Subject 29 Protein Chemistry
10 Plants
22 Legumes-Pulses
 
Description The evolutionary relationship among various BBI isoforms of horsegram was studied. Several inhibitor isoforms have been purified from seeds, cotyledons, leaves and flowers of horsegram and characterized. The data compiled in this thesis describe the purification and characterization of Bowman-Birk inhibitors from various tissues of
horsegram (Dolichos biflorus) plant. The self-association, an anomalous behavior of legume BBIs was analyzed by comparing the primary structure of HGGI-III and HGI-III. The crucial interaction between Lys24 at P1 of the first reactive site of one subunit and the charged residue
Asp76 at the carboxy-terminal of another subunit is the premise of selfassociation in HGI-III. Comparative studies of legume BBI sequences and their self-association behavior indicated that similar interaction could be responsible for the self-association of all reported legume BBIs. Lack of highly immunogenic amino- and carboxy-terminal residues, low Mr and high potency of HGGIs could be an excellent
alternative for use as a cancer chemopreventive agent and developing transgenic insect resistant plants.
 
Date 2004-07
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/351/1/Pradeep_Kumar.pdf
Pradeep, Kumar (2004) Structural Studies of the Proteinase Inhibitors of Germinated Horsegram(Dolichos Biflorus) Seeds. PhD thesis, University of Mysore.