ICAR Research Data Repository for Knowledge Management

Lactic acid bacteria (LAB), Pedίococcus pentosaceus isolated in the laboratory from cheese was tested for its stability and viability under the induced conditions of human gastrointestinal (GI) tract. The culture has shown probiotic properties and has been deposited at MTCC (Microbial Technology Culture Collection Centre) Chandigarh, with an accession No.5151. The culture has a survival of 28.5% at a low pH of 2.0 showing its efficiency to be stable in harsh acidic environment. Shelf-life studies showed survival rate for 21 days under refrigerated condition which makes the culture
suitable for incorporation in fruit juices and other food formulations having low pH. Under simulated human gastrointestinal tract conditions with the particular pH and bile salt mix concentration of jejunum, ileum and large intestine, viable cell count (5 log cfu/ml) was found in the therapeutic minimum range as given by WHO.