Record Details

Detection of methyl parathion using immuno-chemiluminescence based image analysis using charge coupled device.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/533/
http://www.sciencedirect.com/science/journal/09565663
10.1016/j.bios.2005.05.018
 
Title Detection of methyl parathion using immuno-chemiluminescence based image analysis using charge coupled device.
 
Creator Chouhan, R. S.
Vivek Babu, K.
Kumar, M. A.
Neeta, N. S.
Thakur, M. S.
 
Subject 01 Analysis
 
Description A novel method based on immuno-chemiluminescence and image analysis using charge coupled device (CCD) for the qualitative detection of methyl parathion (MP) with high sensitivity (up to 10 ppt) is described. MP antibodies raised in poultry were used as a biological sensing element for the recognition of MP present in the sample. The immuno-reactor column was prepared by packing in a glass capillary column (150 μl capacity) MP antibodies immobilized on Sepharose CL-4B through periodate oxidation method. Chemiluminescence principle was used for the detection of the pesticide. Light images generated during the chemiluminescence reaction were captured by a CCD camera and further processed for image intensity, which was correlated with pesticide concentrations. K3Fe(CN)6 was used as a light enhancer to obtain detectable light images. Different parameters including concentrations of K3Fe(CN)6, luminol, urea H2O2, antibody, addition sequence of reactants and incubation time to obtain best images were optimized. The results obtained by image analysis method showed very good correlation with that of competitive ELISA for methyl parathion detection. Competitive ELISA method was used as a reference to compare the results obtained by CCD imaging.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/533/1/Biosensors_and_Bioelectronics_21_%282006%29_1264-1271.pdf
Chouhan, R. S. and Vivek Babu, K. and Kumar, M. A. and Neeta, N. S. and Thakur, M. S. (2006) Detection of methyl parathion using immuno-chemiluminescence based image analysis using charge coupled device. Biosensors and Bioelectronics, 21 (7). pp. 1264-1271. ISSN 0956-5663