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Heteronuclear saturation transfer difference (HSTD) experiment for detection of ligand binding to proteins.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/535/
http://www.sciencedirect.com/science/journal/00092614
10.1016/j.cplett.2005.12.093
 
Title Heteronuclear saturation transfer difference (HSTD) experiment for detection of ligand binding to proteins.
 
Creator Nagaraja, C.S.
 
Subject 29 Protein Chemistry
 
Description This report presents a modified saturation transfer difference experiment for protein–ligand binding studies. A heteronuclear saturation transfer difference (HSTD) is suggested, where in a hetero atom, such as carbon is utilized for monitoring the binding instead of proton. This method is free from some of the problems associated with proton STD experiment, such as lack of sufficient number of protons at the binding site or crowding of spectra due to smaller chemical shift dispersion. The present method has been demonstrated on three systems namely caffeine–HSA, salicylic acid–HSA and glucose–lysozyme, illustrating the utility of the method.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/535/1/Chemical_Physics_Letters_420_%282006%29_340-346.pdf
Nagaraja, C.S. (2006) Heteronuclear saturation transfer difference (HSTD) experiment for detection of ligand binding to proteins. Chemical Physics Letters, 420 (4-6). pp. 340-346. ISSN 0009-2614