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Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/570/
http://www.sciencedirect.com/science/journal/09254005
10.1016/j.snb.2005.03.018
 
Title Site-directed antibody immobilization on gold substrate for
surface plasmon resonance sensors.
 
Creator Hirlekar Schmid, A.
Stanca, S. E.
Thakur, M. S.
Ravindranathan Thampia, K.
Raman Suri, C.
 
Subject 01 Biotechnology and Bioengineering
 
Description A simple method for the site-directed immobilization of antibodies on gold substrates has been developed for surface plasmon resonance (SPR) applications. A freshly prepared gold surface on a glass slide was modified with Protein A via homobifunctional cross linker dithiobissuccinimide propionate (DSP) to achieve uniform, stable, and sterically accessible antibody coating. The density of the immobilized antibodies bound to Protein A was examined as a function of the protein concentration, buffer pH, buffer type and reaction time. The suggested scheme was also employed for the modification of gold-coated substrates for quartz crystal microbalance (QCM) applications. The modified gold surface was stable for several weeks and the reproducibility was satisfactory. The current fabrication technique could be applied to construct other immunosensors or biochips.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/570/1/Sensors_and_Actuators_B_113_%282006%29_297-303.pdf
Hirlekar Schmid, A. and Stanca, S. E. and Thakur, M. S. and Ravindranathan Thampia, K. and Raman Suri, C. (2006) Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors. Sensors and Actuators B, 113 (1). pp. 297-303. ISSN 0925-4005