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Angiotensin I-Converting Enzyme Inhibitory Peptide Derived from Glycinin, the 11S Globulin of Soybean ( Glycine max)

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Relation http://ir.cftri.com/581/
J.Ag.Food..Chem_01-06
 
Title Angiotensin I-Converting Enzyme Inhibitory Peptide Derived
from Glycinin, the 11S Globulin of Soybean ( Glycine max)
 
Creator Mallikarjun Gouda, K. G.
Lalitha, R. Gowda
Appu Rao, A. G.
Prakash, V.
 
Subject 29 Protein Chemistry
05 Soya bean
 
Description Angiotensin I-converting enzyme (ACE), a dipeptidyl carboxypeptidase, catalyzes the conversion of Angiotensin I to the potent vasoconstrictor Angiotensin II and plays an important physiological role in regulating blood pressure. Inhibitors of angiotensin 1-converting enzyme derived from food proteins are utilized for pharmaceuticals and physiologically functional foods. ACE inhibitory properties of different enzymatic hydrolysates of glycinin, the major storage protein of soybean, have been demonstrated. The IC50 value for the different enzyme digests ranges from 4.5 to 35 Ìg of N2. The Protease P hydrolysate contained the most potent suite of ACE inhibitory peptides. The ACE inhibitory
activity of the Protease P hydrolysate after fractionation by RP-HPLC and ion-pair chromatographywas ascribed to a single peptide. The peptide was homogeneous as evidenced by MALDI-TOF and identified to be a pentapeptide. The sequence was Val-Leu-Ile-Val-Pro. This peptide was synthesized
using solid-phase FMOC chemistry. The IC50 for ACE inhibition was 1.69 ( 0.17 ÌM. The synthetic peptide was a potent competitive inhibitor of ACE with a Ki of 4.5 ( 0.25 10-6 M. This peptide was resistant to digestion by proteases of the gastrointestinal tract. The antihypertensive property of this peptide derived from glycinin might find importance in the development of therapeutic functional foods.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/581/1/J._Agric._Food_Chem._2006%2C_54%2C_4568-4573.pdf
Mallikarjun Gouda, K. G. and Lalitha, R. Gowda and Appu Rao, A. G. and Prakash, V. (2006) Angiotensin I-Converting Enzyme Inhibitory Peptide Derived from Glycinin, the 11S Globulin of Soybean ( Glycine max). Journal of Agricultural and Food Chemistry, 54. pp. 4568-4573.