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Valine Pathway Is More Crucial than Phenyl Propanoid Pathway in Regulating Capsaicin Biosynthesis in Capsicum frutescens Mill.

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Relation http://ir.cftri.com/583/
J.Ag.Food..Chem_03-06
 
Title Valine Pathway Is More Crucial than Phenyl Propanoid
Pathway in Regulating Capsaicin Biosynthesis in
Capsicum frutescens Mill.
 
Creator Prasad, B. C. N.
Gururaj, H. B.
Vinod, Kumar
Giridhar, P.
 
Subject 30 Spices/Condiments
01 Biotechnology and Bioengineering
 
Description Capsaicin, a pungency factor alkaloid of Capsicumfruits, is biosynthesized by enzymatic condensation of vanillylamine, a phenyl propanoid intermediate, and 8-methyl-nonenoic acid, a fatty acid derivative from the leucine/valine pathway by capsaicin synthase. Biotic elicitors, such as aqueous mycelial extracts of Rhizopus oligosporus and Aspergillus niger, and abiotic elicitors, such as salicylic acid
and methyl jasmonate, were incorporated to cell-suspension cultures of Capsicum frutescens.The maximum elicitation of capsaicinoids to the extent of 6-folds was noticed when mycelial extracts of R. oligosporus were incorporated to cell-suspension cultures. The phenyl propanoid intermediates
were elicited to the extent of 3-4-fold, whereas the levels of 8-methyl-nonanoic acid increased 6-fold
in R. oligosporus mycelial-extract-treated cultures. The enzymatic assays of caffeic acid O-methyl
transferases, keto acyl synthase, and capsaicin synthase were performed in elicited and nonelicited
cell-suspension cultures. These enzymes were overexpressed in R. oligosporus mycelial-extracttreated suspension cultures, which was also confirmed by reverse transcriptase polymerase chain reaction studies. This is the first paper on the overexpression of 8-methyl-nonanoic acid leading to
capsaicin biosynthesis using elicitors.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/583/1/J._Agric._Food_Chem._2006%2C_54%2C_6660-6666.pdf
Prasad, B. C. N. and Gururaj, H. B. and Vinod, Kumar and Giridhar, P. (2006) Valine Pathway Is More Crucial than Phenyl Propanoid Pathway in Regulating Capsaicin Biosynthesis in Capsicum frutescens Mill. Journal of Agricultural and Food Chemistry, 54. pp. 6660-6666.