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Hemicelluloses of Ragi (Finger Millet, Eleusine coracana, Indaf-15): Isolation and Purification of an Alkali-Extractable Arabinoxylan from Native and Malted Hemicellulose B

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Relation http://ir.cftri.com/587/
J.Ag.Food..Chem_04-06
 
Title Hemicelluloses of Ragi (Finger Millet, Eleusine coracana,
Indaf-15): Isolation and Purification of an Alkali-Extractable
Arabinoxylan from Native and Malted Hemicellulose B
 
Creator Subba Rao, M. V. S. S. T.
Muralikrishna, G.
 
Subject 05 Ragi (Finger Millet)
28 Polysaccharide Chemistry
 
Description Hemicelluloses (A and B) were isolated from an Indo-African hybrid variety of finger millet (ragi,Eleusine coracana) by extracting the starch-free residue with 10% sodium hydroxide under a continuous stream of nitrogen, and changes in their sugar composition during malting for 96 h were
studied. Hemicellulose B, obtained in higher yield from both native (N) and malted (M) flours, was found to be completely soluble in water, richer in uronic acid, and more viscogenic than hemicelullose A. Fractional precipitation of hemicellulose B by ammonium sulfate resulted in four precipitable fractions (F-60, F-70, F-80, and F-100) and a nonprecipitable (NP) fraction varying in their yield and arabinose,xylose, galactose, and glucose contents. A progressive increase in the pentose-to-hexose ratio (P:
H) from 0.42:1.0 in F-60 to 1.94:1.0 in NP was observed in native hemicellulose B fractions; however,in malted hemicellulose B the P:H ratio increased from 0.43:1.0 in F-60 to 1.56:1.0 in F-80 and then decreased to 1.13:1.0 in NP. The major fraction, F-70 (N, 44.5%; M, 38.5%), was separated into eight subfractions on DEAE-cellulose by successive elution with water, ammonium carbonate (AC)
(0.1, 0.2, and 0.3 M AC), and sodium hydroxide (0.1 and 0.2 M) differing in their yield and neutral sugar composition. The purity of the major glucuronoarabinoxylan fraction (0.1 M AC eluted) was ascertained by Sepharose CL-4B, HPSEC, cellulose acetate, and capillary electrophoresis methods.
A significant decrease in the molecular mass of arabinoxylan from 1200 to 1120 kDa upon malting for 96 h is an indication of cell wall degradation by the inducible cell wall degrading enzymes.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/587/1/J._Agric._Food_Chem._2006%2C_54%2C_2342-2349.pdf
Subba Rao, M. V. S. S. T. and Muralikrishna, G. (2006) Hemicelluloses of Ragi (Finger Millet, Eleusine coracana, Indaf-15): Isolation and Purification of an Alkali-Extractable Arabinoxylan from Native and Malted Hemicellulose B. Journal of Agricultural and Food Chemistry, 54. pp. 2342-2349.