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A Spectroscopic Study of the Interaction of Nigerloxin, a Fungal Metabolite, with Serum Albumin

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Lipid-01-04
 
Title A Spectroscopic Study of the Interaction of Nigerloxin, a Fungal Metabolite, with Serum Albumin
 
Creator Sekhar Rao, K. C.
Appu Rao, A. G.
Sattur, A. P.
 
Subject 29 Protein Chemistry
04 Fungi
 
Description Nigerloxin [2-amido-3-hydroxy-6-methoxy-5-tethyl-4-(prop-1'-enyl) benzoic acid], a fungal metabolite, is an inhibitor of lipoxygenase and aldose reductase with free radical-scavenging properties. The interaction of nigerloxin with avine serum albumin (BSA) was investigated using fluorescence spectroscopy and circular dichroic measurements. The fuorescence of BSA was
quenched following interaction with nigerloxin, and this property was used to generate a binding
constant. The estimated association constant was 1.01± 0.2 x 06 M-1. Job's method of continuous variation indicated that nigerloxin formed a 1:1 ±t 0.1 complex with BSA. To understand the nature of the interaction, the variance in the association constant as a function of temperature in the range of 14-45°C was used to calculate the thermodynamic parameters. The thermodynamic parameters at
27°C derived from the mass action lot and van't Hoff's plot were as follows: ∆G = -8.2t±0.1. kcal/mol,
∆H = 0 kcal/mol, and ∆S = 27.5 ±t 0.4 cal/mol/K where ∆G is free energy, ∆H is enthalpy, and ∆S is
entropy). Increasing ionic strength did not favor interaction. Circular dichroic measurements revealed that the interaction of nigerloxin with BSA did not lead to changes in the secondary structure of the protein.
The reversibility of the interaction verified by the dilution method was found to be reversible. These
measurements suggest that partial hydrophobic and partial ionic bonding play a role in the interaction
of nigerloxin with BSA.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1242/1/lipids_39__2__173-177.pdf
Sekhar Rao, K. C. and Appu Rao, A. G. and Sattur, A. P. (2004) A Spectroscopic Study of the Interaction of Nigerloxin, a Fungal Metabolite, with Serum Albumin. Lipids, 39 (2). pp. 173-177.