Record Details

Depolymerized products of chitosan as potent inhibitors of tumor-induced angiogenesis.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/1261/
http://www.elsevier.com/locate/bba
10.1016/j.bbagen.2004.11.009
 
Title Depolymerized products of chitosan as potent inhibitors
of tumor-induced angiogenesis.
 
Creator Harish Prashanth, K. V.
Tharanathan, R. N.
 
Subject 28 Meat, Fish & Poultry
 
Description Water-soluble low-molecular weight chitosan (LMWC) and chitooligosaccharides (COs) were obtained from chitosan (16% Nacetylation) by depolymerization induced by potassium persulfate under nitrogen atmosphere for 2 h. They were characterized by IR, X-ray, HPLC and 13C-NMR. Splitting of C3/C5 signals in the latter indicated a newer conformation, and also showed prominence of acetyl groups in LMWC, may be due to cleavage between two consecutive deacetylated residues. Molecular weight of LMWC, determined by HPSEC,
showed a single peak of ~37 kDa. HPLC analysis of the solvent-extracted fraction revealed COs enriched with pentamer, hexamer and higher oligomers. The effect of LMWC and COs on the growth of Ehrlich ascites tumor (EAT) cells and tumor-induced neovascularization was studied. COs (50 Ag) were more effective compared to LMWC (100 Ag) and proved to be potent angioinhibitory and antitumor compounds,
as shown by inhibition of angiogenesis and inducing apoptosis as a function of DNA fragmentation.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/1261/1/Biochimica_et_Biophysica_Acta_1722_%282005%29_22-_29.pdf
Harish Prashanth, K. V. and Tharanathan, R. N. (2005) Depolymerized products of chitosan as potent inhibitors of tumor-induced angiogenesis. Biochimica et Biophysica Acta, 1722 (1). pp. 22-29. ISSN 0304-4165