ICAR Research Data Repository for Knowledge Management
Molecular understanding of aluminum-induced topological changes in(\[CCG_12\]) triplet repeats: relevance to neurological disorders.
IR@CSIR-CFTRI
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/1266/
http://www.bba-direct.com BBA-2002-1 |
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| Title |
Molecular understanding of aluminum-induced topological changes in(\[CCG_12\]) triplet repeats: relevance to neurological disorders.
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| Creator |
Latha, K. S.
Anitha, S. Jagannatha Rao, K. S. Viswamitra, M. A. |
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| Subject |
03 Biochemistry & Molecular Biology
02 Evolution & genetics |
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| Description |
Recent studies have shown that gene mutations are involved in the pathology of neurological disorders. CCG repeats cause genetic instability and are localized at the 5Vend of the non-coding regions of the FMR1 gene in fragile X syndrome. Our studies for the first time showed that aluminum (Al) levels were elevated in the serum samples of fragile X syndrome and also provide evidence for the interaction of aluminum with (\[CCG_12\])-repeats. Circular dichroism spectroscopic studies of (\[CCG_12\])indicated B-DNA conformation and in the presence of Al (\[10^5\]M) CCG repeats attained Z-DNA conformation. Further spectroscopic studies, which included melting profiles, ethidium bromide binding patterns and interaction of Z-DNA specific polyclonal antibodies confirmed the Z-conformation in (CCG)12-repeats in the presence of Al (\[10^5\]M). It is interesting to mention that Al-induced Z-conformation is stable even after the total removal of Al from CCG by desferoximine, a chelating drug. This is the first report to proof the role of Al in modulating the DNA (CCG repeats) topology and this information provides a clue about the possible involvement of Al at a molecular level in neurological/neurodegenerative disorders. |
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| Date |
2002
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/1266/1/Biochimica_et_Biophysica_Acta_1588_%282002%29_56-64.pdf
Latha, K. S. and Anitha, S. and Jagannatha Rao, K. S. and Viswamitra, M. A. (2002) Molecular understanding of aluminum-induced topological changes in(\[CCG_12\]) triplet repeats: relevance to neurological disorders. Biochimica et Biophysica Acta, 1588. pp. 56-64. ISSN 0925-4439 |
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