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Preferential interaction of denaturants with rice bran lipase

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IJBM-01-96
 
Title Preferential interaction of denaturants with rice bran lipase
 
Creator Rajeshwara, A. N.
Gopalakrishna, K. N.
Prakash, V.
 
Subject 01 Rice
29 Protein Chemistry
16 Enzyme Chemistry
 
Description The catalytic stability of rice bran lipase has been determined in the presence of three denaturants viz, urea, GuHCI and GuHSCN. The enzyme is completely inactive above 7 M urea, 4 M GuHCI and 2 M GuHSCN concentration. The extent of denaturant interaction has been determined by the partial specific volume measurements of the enzyme. The preferential interaction parameter (~3) has values of 0.042, 0.064 and 0.075 g/g, and the denaturation volume changes are -180, -240 and -270 ml/mol in presence of 8 M urea, 6 M GuHCI and 3 M GuHSCN, respectively. The experimental values of number of denaturant molecules
bound (A3) are 0.418, 0.566 and 0.320 g/g and the calculated values are 0.321, 0.511 and 0.632 g/g in presence of 8 M urea, 6 M GuHCI and 3 M GuHSCN, respectively. Fluorescence emission measurements indicated a decrease in the fluorescence emission intensity and a red shift in the emission maximum as the denaturant concentration is increased indicating the gradual exposure
of aromatic chromophores. The instability of the enzyme in the presence of these denaturants has been indicated by a decreased value of apparent thermal denaturation temperature (Tm) of the enzyme from a control value of 67°C. The results obtained in the present study explain the extent of inactivation/stability of rice bran lipase in presence of these denaturants at different concentrations.
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1276/1/International_Journal_of_Biological_Macromolecules_19_%281996%29_1-7.pdf
Rajeshwara, A. N. and Gopalakrishna, K. N. and Prakash, V. (1996) Preferential interaction of denaturants with rice bran lipase. International Journal of Biological Macromolecules, 19. pp. 1-7.