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First evidence on induced topological changes in supercoiled DNA by an aluminium D-aspartate complex

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Relation http://ir.cftri.com/1284/
JBIC-01-03
 
Title First evidence on induced topological changes in supercoiled DNA by an aluminium D-aspartate complex
 
Creator Ms., Bharathi
Jagannatha Rao, Kosagi Sharaf
Stein, Reuven
 
Subject 13 Molecular Biochemistry
 
Description Aluminium is a debatable and suspected etiological
factor in neurodegenerative disorders. Aluminium
–amino acid complexes also play an important role
in the complex biology of the metal. Recent reports
indicate the presence of D-aspartate and D-glutamate in
aging brain, human breast tumors, core amyloid plaques
and neurofibrillary tangles of Alzheimer’s brain. This
stereoinversion from the L- to the D-enantiomer is
enhanced by Al. Further, the observation that Al is
localized in the chromatin region encouraged the present
study of the interaction of Al–amino acid complexes
with DNA. This study used circular dichroism of
supercoiled DNA and showed that Al–D-Asp caused a
native B-DNA to C-DNA conformational change, while
Al–L-Asp, Al–L-Glu and Al–D-Glu did not alter the
native B-DNA conformation. This differential DNA
binding property of Al–amino acid complexes is
assigned to the stereoisomerism and chirality of the
complexes. Interestingly, polyamines like spermine further
induced an asymmetric condensation of the ‘‘limit
C-motif’’ induced by Al–D-Asp to a Y-DNA. The results
were supported by computer modeling, gel studies and
ethidium bromide binding. We also propose a mechanism
of Al–D-Asp binding and its ability to modulate
DNA topology.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/1284/1/Journal_of_Biological_Inorganic_Chemistry_8%282003%29_823-830.pdf
Ms., Bharathi and Jagannatha Rao, Kosagi Sharaf and Stein, Reuven (2003) First evidence on induced topological changes in supercoiled DNA by an aluminium D-aspartate complex. Journal of Biological and Inorganic Chemistry, 8. pp. 823-830.