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Modulation of K+ transport across synaptosomes of rat brain by synthetic pyrethroids

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Relation http://ir.cftri.com/1288/
JNS-01-97
 
Title Modulation of K+ transport across synaptosomes of rat brain by synthetic pyrethroids
 
Creator Valeswara Rao, Gazula
Jagannatha Rao, Kosagi Sharaf
 
Subject 26 Pesticide Chemistry
 
Description Potassium transport across synaptosomes was studied under the influence of two synthetic pyrethroids, (Permethrin, without the cyano group) and Cypermethrin (CPM, with the cyano group). Synaptosomes were isolated from rat brain cerebral cortex and incubated with 40 PM of PM and CPM for 15 min at 37°C. K’ release was monitored by a K’-sensitive electrode. CPM caused more K+ release from synaptosomes compared to PM. K+ transport is regulated by Naf-K’-ATPase, K+-ATPase and Kt channels. To understand the mode
of action, synaptosomes were preincubated with 9.5X l0-5 M ouabain (inhibitor of Na’-K+-ATPase), 1.7X lOA* M N-ethylmaleamide (K’-ATPase inhibitor), and 9.5X 10-5 M quine sulfate (K+-channel blocker) for 15 min at 37°C. In the presence of ouabain and N-ethylmaleamide, PM- and CPM-induced K’ release was decreased and in the presence of quinine sulfate, there was no release of K’.
Furthermore, the studies indicated that PM and CPM significantly decreased K’ uptake.
 
Date 1997
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1288/1/Journal_of_Neurological_Sciences_147_%281997%29_127-133.pdf
Valeswara Rao, Gazula and Jagannatha Rao, Kosagi Sharaf (1997) Modulation of K+ transport across synaptosomes of rat brain by synthetic pyrethroids. Journal of Neurological Sciences (147). pp. 127-133.