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Hepatic binding proteins translocating azo dye carcinogen metabolites from cytoplasm into nucleus in rats.

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FCT-01-04
 
Title Hepatic binding proteins translocating azo dye carcinogen
metabolites from cytoplasm into nucleus in rats.
 
Creator Srinivasan, K.
Bhargava, M. M.
 
Subject 11 Food Biochemistry
 
Description When liver cytosol prepared from rats administered [14C]-30-Methyl-N,N-dimethyl-4-aminoazobenzene was subjected to Sephadex
gel chromatography, four peaks of radioactivity containing proteins (Peak-I–IV) and one peak devoid of protein (Peak-V) were
obtained. Translocation of azo dye metabolites from these various cytosolic fractions into nucleus was studied in an in vitro system
and a maximum of about 10% of the radioactivity associated with a particular cytosolic fraction (Peak-II) could translocate into
the nuclei. Radioactivity (%) translocated did not increase upon addition of excess nuclei. Passage of this protein fraction through
an immobilized protease column reduced the azo dye metabolite translocation by 65%, concomitant with the degradation of
proteins. Translocation was not observed with protein-free metabolites extracted from this cytosolic fraction; addition of proteins
corresponding to peak-II from normal rat liver cytosol significantly restored the metabolite translocation. This observation suggests
that specific cytosolic proteins are involved in the translocation of azo dye carcinogen metabolites from liver cytoplasm into the
nucleus. When the liver cytosolic proteins corresponding to this fraction (Peak-II) were iodinated with 125I-iodine and incubated
with purified nuclei, translocation of three specific proteins into nucleus was observed as seen by SDS–PAGE and fluorography of
nuclear proteins. Covalent binding of azo dye metabolites to DNA was not observed when cytosolic peak-II fraction containing
azo dye metabolites was incubated with isolated liver DNA instead of liver nuclei. This suggests that the interaction of azo dye
metabolites with nuclear macromolecules necessitate further prior processing which actually may occur in the nucleus.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/1290/1/Food_and_Chemical_Toxicology_42%283%29_2004_503-.pdf
Srinivasan, K. and Bhargava, M. M. (2004) Hepatic binding proteins translocating azo dye carcinogen metabolites from cytoplasm into nucleus in rats. Food and Chemical Toxicology, 42. pp. 503-508.