Record Details

Methyl jasmonate modulated biotransformation of phenylpropanoids to vanillin related metabolites using Capsicum frutescens root cultures

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/1294/
PPB
 
Title Methyl jasmonate modulated biotransformation of phenylpropanoids to vanillin related metabolites using Capsicum frutescens root cultures
 
Creator Suresh, B.
Ravishankar, G. A.
 
Subject 23 Vegetables
01 Biotechnology and Bioengineering
 
Description Normal root cultures of Capsicum frutescens biotransform externally fed precursors, like caffeic acid and veratraldehyde, to vanillin and other related metabolites. The bioconversion of caffeic acid to further metabolites—viz. vanillin, vanillylamine, vanillic acid—was shown to
be elicited by treating the cultures with 10 µM methyl jasmonate (MJ). Root cultures treated with MJ accumulated (1.93 times) more of vanillin (20.2 µM on day-3) than untreated ones.A concomitant increase in enzymatic activity of caffeic acid O-methyl transferase (CAOMT,
EC 2.1.1.68) was obtained in MJ treated cultures, compared to untreated cultures. After 24 h ofMJ treatment, a 13.7-fold increase in CAOMT activity was recorded in root cultures of C. frutescens. Cultures treated with veratraldehyde accumulated more vanillin (78 µM) than caffeic acid fed cultures, 6 days after precursor addition. Capsaicin did not accumulate even after addition of precursors. The efficiencies of biotransformation
with caffeic acid and veratraldehyde were 2.2% and 9% with respect to vanillin formation, indicating a possible diversion of the phenylpropanoid pathway towards other secondary metabolites.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1294/1/Plant_Physiology_and_Biochemistry_43_%282005%29_125-131.pdf
Suresh, B. and Ravishankar, G. A. (2005) Methyl jasmonate modulated biotransformation of phenylpropanoids to vanillin related metabolites using Capsicum frutescens root cultures. Plant Physiology and Biochemistry, 43. pp. 125-131.