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Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1649/
BioPlan-01-08
 
Title Direct organogenesis from leaf explants of Stevia rebaudiana
and cultivation in bioreactor
 
Creator Sreedhar, R. V.
Venkatachalam, L.
Thimmaraju, R.
Bhagyalakshmi, N.
Narayan, M. S.
Ravishankar, G. A.
 
Subject 06 Trees And Shrubs
05 Tissue Culture
 
Description Shoot buds were induced directly on either side of midrib from adaxial surface of immature leaf explants in Stevia
rebaudiana Bertoni five weeks after culturing in Murashige and Skoog’s nutrient medium supplemented with 8.88 μM
of N6-benzylaminopurine and kinetin ranging from 4.65 to 6.98 μM. Immature leaves of 0.6 to 1 cm were found to
produce best response (93 %) with a highest number of 4.93 shoot buds per explant. For elongation of regenerated shoot
buds, MS medium supplemented with 30 g dm-3 sucrose and indole-3-butyric acid (IBA) ranging from 4.92 to 7.38 μM
were found most suitable. The medium was further modified to suit bioreactor cultivation of regenerated shoots wherein
the use of two-fold MS salts and 60 g dm-3 sucrose resulted in a high biomass yield of 50.68 g dm-3 (m/v) accounting for
about 590 micro-cuttings in three weeks. Best rooting of micro-cuttings occurred in half strength MS medium
supplemented with IBA ranging from 4.92 to 7.38 μM, 15 g dm-3 sucrose and gelled with 0.8 % agar. Rooted plants
were successfully established in substrate containing sand, Vermicompost and garden soil in equal proportions and
grown in greenhouse. This is the first report on direct shoot regeneration from Stevia leaves.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1649/1/BIOLOGIA_PLANTARUM_52_%282%29__355-360%2C_2008.pdf
Sreedhar, R. V. and Venkatachalam, L. and Thimmaraju, R. and Bhagyalakshmi, N. and Narayan, M. S. and Ravishankar, G. A. (2008) Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biologia Plantarum, 52 (2). pp. 355-360.