Record Details

Evaluation of a new medium for rapid enumeration of yeasts and molds in food

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/1948/
JFST-14-88
 
Title Evaluation of a new medium for rapid enumeration of yeasts and molds in food
 
Creator Venkatasubbaiah, P.
Dwarakanath, C. T.
 
Subject 08 Food technology
04 Yeast
 
Description Conventional methods of enumeration of yeasts and moulds in foods and feeds involve long incubation periods (5 days) at ambient temp. A rapid method was evolved using a more nutritious medium (DYPBS medium) consisting of malt extract or yeast extract, beef extract, dextrose, peptone and NaCl (pH 6.1) and incubation at 30 degree C for 48 h. Chlorotetracycline and chloramphenicol at 100 p.p.m. levels were used for controlling bacterial growth. Comparative studies carried out using 2 storage fungi (Aspergillus sp. and Rhizopus sp.) and 3 strains of yeasts (Saccharomyces cerevisiae, Hansenula anamola and Torulopsis holmii) with 6 different conventional media indicated better recovery using the DYPBS medium. Recovery of added Aspergillus niger spores to different food grains was high with DYPBS medium, and compared to other media gave highest recoveries from rice, wheat, Bengal gram and sorghum. The modified method was also found to help in reviving cultures that had been subjected to heat, chill, freeze or osmotic stress. [From En summ.]
 
Date 1988
 
Type Article
PeerReviewed
 
Identifier Venkatasubbaiah, P. and Dwarakanath, C. T. (1988) Evaluation of a new medium for rapid enumeration of yeasts and molds in food. Journal of Food Science and Technology, 25 (1). 4-6,9. ISSN 0022-1155