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Digital image processing-an alternate tool for monitoring of pigment levels in cultured cells with special reference to green alga Haematococcus pluvialis.

IR@CSIR-CFTRI

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Title Digital image processing-an alternate tool for monitoring of pigment levels in cultured cells with special reference to green alga Haematococcus pluvialis.
 
Creator Sandesh Kamath, B.
Ms, Shalini Chidambar
Brinda, B. R.
Kumar, M. A.
Sarada, R.
Ravishankar, G. A.
 
Subject 11 Food Biochemistry
08 Food Chemistry
 
Description A method for analyzing carotenoid content in Haematococcus pluvialis, a green alga was developed using digital image processing (DIP) and an artificial neural network (ANN) model. About 90 images of algal cells in various phases of growth were processed with the tools of DIP. A good correlation of R(2)=0.967 was observed between carotenoid content as estimated by analytical method and DIP. Similar correlation was also observed in case of chlorophyll. Since the conventional methods of carotenoid estimation are time consuming and result in loss of pigments during analysis, DIP method was found to be an effective online monitoring method. This method will be useful in measurement of pigments in cultured cells.
 
Date 2005
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/1966/1/BiosensBioelectron21768-773.pdf
Sandesh Kamath, B. and Ms, Shalini Chidambar and Brinda, B. R. and Kumar, M. A. and Sarada, R. and Ravishankar, G. A. (2005) Digital image processing-an alternate tool for monitoring of pigment levels in cultured cells with special reference to green alga Haematococcus pluvialis. Biosensors and Bioelectronics, 21. pp. 768-773. ISSN 0956-5663