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Efficient clonal propagation method for Decalepis hamiltonii, an endangered shrub, under the influence of phloroglucinol.

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Title Efficient clonal propagation method for Decalepis hamiltonii, an endangered shrub, under the influence of phloroglucinol.
 
Creator Gururaj, H. B.
Giridhar, P.
Ravishankar, G. A.
 
Subject 23 Vegetables
01 Plant Cell
 
Description A highly efficient two stage protocol was developed for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phloroglucinol (PG) had synergistic effect on shoot multiplication when added with N6-benzyladenine and gibberellic acid. This protocol uses PG for both multiple shoot induction from nodal explants, elongation of primary shoots and initiation of adventitious shoot formation from primary shoots, which was more in presence of triacontanol (TRIA). Maximum number of shoots per culture was observed on the medium containing N6-benzyladenine (1.1 microM; BA), GA3 (5.8 microM) and PG (800 microM). Sub-culturing of the shoots onto MS medium containing optimum concentration of BA (5.6 microM), PG (200 microM) and TRIA (0.011 microM) produced elongated shoots along with secondary shoot formation. The long shoots were rooted on alpha-naphthalene acetic acid (5.38 microM; NAA) and PG (400 microM) containing medium. The rooted plantlets were hardened and their field survival rate was 80-90%.
 
Date 2004
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2039/1/Indian_Journal_of_Experimental_Biology_2004_42_424-428.pdf
Gururaj, H. B. and Giridhar, P. and Ravishankar, G. A. (2004) Efficient clonal propagation method for Decalepis hamiltonii, an endangered shrub, under the influence of phloroglucinol. Indian Journal of Experimental Biology, 42 (4). pp. 424-4288. ISSN 0019-5189