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In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents

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Relation http://ir.cftri.com/2118/
CS-02-01
 
Title In vitro rooting of Decalepis hamiltonii
Wight & Arn., an endangered shrub,
by auxins and root-promoting agents
 
Creator Obul Reddy, B.
Giridhar, P.
Ravishankar, G. A.
 
Subject 06 Trees And Shrubs
05 Tissue Culture
 
Description In the present study micropropagation of an endangered
shrub, Decalepis hamiltonii Wight & Arn.,
optimization and standardization of efficient methods
for rooting of in vitro-derived micro-shoots by using
phloroglucinol (PG), activated charcoal and CoCl2
have been achieved. Transfer of in vitro-derived shoots
to MS medium supplemented with 8.8 mM indole-3-
butyric acid (IBA) and 1.43 mM indole-3-acetic acid
resulted in root induction. In another treatment, IBA
was found to be a good root-promoting agent, wherein
dipping of explants in 4.4 μM IBA for 30 min and
subsequent inoculation on MS basal medium was also
beneficial for root induction. Successful field transfer
was accomplished in rooted plantlets.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2118/1/CURRENT_SCIENCE%2C_VOL._81%2C_NO._11%2C_10_DECEMBER_2001CURRENT_SCIENCE%2C_VOL._81%2C_NO._11%2C_10_DECEMBER_2001_1479.pdf
Obul Reddy, B. and Giridhar, P. and Ravishankar, G. A. (2001) In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents. Current Science, 81 (11). pp. 1479-1482.