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Membrane changes in rat erythrocyte ghosts on ghee feeding.

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MCB-12-00
 
Title Membrane changes in rat erythrocyte ghosts on ghee feeding.
 
Creator Niranjan, T. G.
Krishnakantha, T. P.
 
Subject 19 Lipids-oils/fats
 
Description Alterations in membrane lipid composition is known to result in functional and structural changes in the membrane, and dietary lipids play an important role in this change. It was of interest to study the influence of ghee feeding to the rat on membrane structure and function. The activities of membrane bound enzymes Na+ K+ ATPase and Acetylcholinesterase were studied as an index of membrane changes. Male weanling rats were fed 2.5% fresh or thermally oxidized ghee in the diet for a period of 8 weeks. The control rats were fed groundnut oil. A decrease of 28% in the membrane fluidity of erythrocyte ghost membranes was observed in the oxidized ghee fed group at 37 degrees C, by fluorescence polarization measurements using 1,6-Diphenyl-1,3,5-hexatriene as a probe. The activities of Na+ K+ ATPase and Acetylcholinesterase showed an increase of 65 and 200% respectively after feeding oxidized ghee (2.5%). Also changes in Na+, K+ and ATP kinetics were observed in these rats. Increased membrane lipid peroxidation (80%) and C/PL ratio (11%) in the oxidized ghee fed group was observed. Marginal changes in the fatty acid composition were also seen. Further, an increase in the osmotic fragility of erythrocytes was observed in the oxidized ghee fed rats. It is inferred from these experiments that consumption of oxidized ghee with the diet affects the erythrocyte ghost membrane structure and function at 2.5% level, whereas consumption of fresh ghee has no effect on the erythrocyte membrane.
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2171/1/Mol._Cellular_Biochem_203%281-2%29_2000.pdf
Niranjan, T. G. and Krishnakantha, T. P. (2000) Membrane changes in rat erythrocyte ghosts on ghee feeding. Molecular and Cellular Biochemistry, 204 (1-2). pp. 57-63. ISSN 0300-8177