ICAR Research Data Repository for Knowledge Management
Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels.
IR@CSIR-CFTRI
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http://ir.cftri.com/2198/
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| Title |
Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels.
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| Creator |
Suresh Babu, C. V.
Dubey, A. K. Kini, R. Umesh Kumar, S. Karanth, N. G. |
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| Subject |
16 Enzyme Chemistry
25 Sugar/Starch/Confectionery |
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| Description |
A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. The reagent reacts with the reducing group of glucose released by glucoamylase from the substrate starch. Using the reaction, production of glucoamylase by Aspergillus niger was detected on 10% IEF gels within a pH range of 2.5-9.5. Since the method can detect raw and gelatinized starch activities of glucomylase associated with 1 microg protein, it will be useful for enzyme engineering studies that involve screening of various mutations.
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/2198/1/Electrophoresis%201999%2C%2020%2C%20483-485.pdf
Suresh Babu, C. V. and Dubey, A. K. and Kini, R. and Umesh Kumar, S. and Karanth, N. G. (1999) Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels. Electrophoresis, 20 (3). pp. 483-485. ISSN 0173-0835 |
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