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Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/2212/
EMT-01-89
 
Title Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin
 
Creator Joshi, M. S.
Gowda, L. R.
Katwa, L. C.
Bhat, S. G.
 
Subject 19 Yeast
01 Biotechnology and Bioengineering
 
Description Kluyveromyces fragilis, the lactose-fermenting yeast, was made permeable to the disaccharide lactose using the surfactant digitonin. Optimal beta-galactosidase activity was observed when the cells were treated with 0.1% digitonin at room temp. for 30 min. Activity measured in permeabilized cells was 400-500x greater than in untreated cells and 25% more than in cell-free extracts prepared by toluene autolysis. Permeabilized cells rapidly hydrolysed lactose in whole milk and sweet whey to glucose and galactose. Glucose was not further degraded by permeabilized cells; untreated cells metabolized the glucose to ethanol.
 
Date 1989
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/2212/1/Enzyme%20Microbial%20Tech_1989.pdf
Joshi, M. S. and Gowda, L. R. and Katwa, L. C. and Bhat, S. G. (1989) Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin. Enzyme and Microbial Technology, 11 (7). pp. 439-443. ISSN 0141-0229