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Detergent permeabilized yeast cells as the source of intracellular enzymes for estimation of biomolecules.

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EMT-01-93
 
Title Detergent permeabilized yeast cells as the source of intracellular enzymes for estimation of biomolecules.
 
Creator Bhat, N.
Naina, N. S.
Gowda, L. R.
Bhat, S. G.
 
Subject 19 Yeast
05 Enzymes
 
Description The feasibility of using permeabilized whole cells as a source of intracellular enzymes instead of isolated expensive enzymes for the estimation of biomolecules has been studied. Alcohol dehydrogenase (ADH), glucose-6-phosphate dehydrogenase (G6PDH), hexokinase (HK), and beta-galactosidase (beta-GAL) activities of cetyltrimethylammonium bromide (CTAB)-permeabilized whole yeast cells were employed to estimate ethyl alcohol, glucose, and lactose. The method using permeabilized cells was comparable to that of isolated enzymes and was applicable for the estimation of these analytes in complex samples such as blood, milk, and fermented samples. The usefulness of permeabilized cells as a single source of more than one enzyme required for coupled enzyme assays was demonstrated.
 
Date 1993
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2284/1/Enzyme_and_Microbial_Technology%2C_Volume_15%2C_Issue_9%2C_September_1993%2C_Pages_796-800.pdf
Bhat, N. and Naina, N. S. and Gowda, L. R. and Bhat, S. G. (1993) Detergent permeabilized yeast cells as the source of intracellular enzymes for estimation of biomolecules. Enzyme and Microbial Technology, 15 (9). pp. 796-800. ISSN 0141-0229